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Method of Spectrophotometric Microan

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Method of Spectrophotometric Microanalysis Based on HRP/PET Self-assembly Film

Abstract: Horseradish peroxidase monolayer was assembled on the surface of PET-CO2 ̄ substrate. The reaction kinetics of HRP/PET film and H2O2 in micro reactor was studied using improved spectrophotometer. The relative activity of self-assembly HRP/PET film still remains above 80% after storing for 150 days at 4℃. When applied to determination of H2O2 in sample, the recoveries of H2O2 are 96.5%~101.1%. 作 者: Xiu Ming JIANG    Zhi Chun CHEN    Shao Ming YANG    Han Feng LIN    Xian Fu LIN   作者單位: Xiu Ming JIANG(Chemistry Department of Zhejiang University, Hangzhou ,310027;Chemistry Department of Zhengzhou Institute of Technology, Zhengzhou, 450052)

Zhi Chun CHEN,Shao Ming YANG,Han Feng LIN,Xian Fu LIN(Chemistry Department of Zhejiang University, Hangzhou ,310027) 

期 刊: 中國化學快報(英文版)  ISTICSCI   Journal: CHINESE CHEMICAL LETTERS  年,卷(期): 2004, 15(5)  分類號:   Keywords: Electrostatic self-assembly film    horseradish peroxidase (HRP)    spectrophotometry    atomic force microscope (AFM)